Effect of RNA Gas5 on Colorectal Cancer Cell Lines
Posted: Tuesday, April 3, 2018
The overexpression of long noncoding RNA (lncRNA) Gas5 inhibited the proliferation, migration, and invasion of colorectal cancer cells but promoted apoptosis of these cells, according to research published by Jin Li, MD, of the Huai’an First People’s Hospital, Nanjing Medical University, China, and colleagues, in Cancer Cell International. However, the researchers noted further study on a larger sample size is needed to confirm their findings.
Colorectal cancer and normal tissues were collected from 126 patients with colorectal cancer, and normal intestinal epithelial cell line FHC and colorectal cancer cell lines (HCT-8, HT-29, HCT-116, and SW-480) were prepared.
Gas5 expression was found to be lower in colorectal cancer tissues than in the adjacent normal tissues and was also lower in colorectal cancer cell lines than normal intestinal epithelial cell line FHC. In addition, Gas5 expression exerted a negative control in tumor size and TNM staging, the researchers reported. Gas5 mRNA and protein expression did not appear to be correlated to age, gender, tumor site, tumor differentiation, or lymph node metastasis (P > .05). Finally, the researchers found that those with low Gas5 expression had significant lower median survival time than did those with high Gas5 expression (24 vs. 29 months).
“Our results demonstrate that Gas5 plays a crucial role in the clinicopathological features and prognosis of colorectal cancer, which may provide significant clues for further clinical practice,” Dr. Li and colleagues concluded.