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AACR 2022: Use of Tumor-Derived DNA From Urine and Plasma Samples to Predict Outcomes in Bladder Cancer

By: Kayci Reyer
Posted: Tuesday, April 12, 2022

According to research presented at the American Association for Cancer Research (AACR) Annual Meeting 2022 (Abstract 1275/22), DNA derived from muscle-invasive bladder tumors may be more likely to appear in urine samples than in plasma samples. Relationships between the clearance of tumor-derived DNA levels and treatment outcomes were noted across sample types, in this analysis via deep targeted sequencing of both urine and plasma samples.

“Interestingly, we show that tumor-derived DNA dynamics in urine supernatants might better reflect the local tumor environment, and plasma-based tumor-derived DNA better reflects outcomes following surgery (ie, disease dissemination),” noted Lars Dyrskjøt, PhD, of Aarhus University Hospital, Denmark, and colleagues.

The study included 92 patients with localized muscle-invasive bladder cancer who had undergone treatment with neoadjuvant chemotherapy followed by radical cystectomy. Precystectomy samples of plasma (n = 167), urine supernatants (n = 281), and urine pellets (n = 114) were collected and analyzed via deep targeted sequencing. An additional 288 plasma samples associated with 56 patients from a previous study were evaluated.

An association was noted between tumor-derived DNA levels in pairs of supernatants and pellets from urine samples, with higher DNA levels occurring in pellets. About 20% of plasma samples had observable DNA versus 63% and 68% of supernatant and pellet samples, respectively. The clearance of tumor-derived DNA in urine supernatants appeared to be significantly associated with neoadjuvant chemotherapy outcomes; however, clearance of tumor-derived DNA in plasma samples was significantly associated with recurrence-free survival (P < .001) but not for urine supernatants (P = .06) and urine pellets (P = .53).

Disclosure: The study authors reported no conflicts of interest.

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