Connecting LncRNA Molecule to Development of Head/Neck Squamous Cell Carcinoma
Posted: Wednesday, November 20, 2019
The long noncoding RNA (LncRNA) LINC00460 was found to promote the epithelial-to-mesenchymal transition in head and neck squamous cell carcinomas by aiding peroxiredoxin-1 (PRDX1) in its entry to the nucleus. Jiang et al of Weifang Medical University, China, published the results of their research in the Journal of Experimental & Clinical Cancer Research.
“LINC00460 and PRDX1 are promising candidate prognostic predictors and potential targets for cancer therapy for head and neck squamous cell carcinoma,” concluded the authors.
The team assessed the function of LINC00460 in various epithelial cancer lines. They were able to localize its subcellular location using a combination of nuclear/cytoplasmic fractionation and fluorescence in situ hybridization. In addition, RNA pull-down assays and immunoprecipitation assays were helpful in understanding the function of LINC00460 in cancer cell progression.
LINC00460 was found to induce the epithelial-to-mesenchymal transition in cancer cells and enhance cell proliferation and metastasis. The primary location of LINC00460 was the cytoplasm, where it interacted with PRDX1 to allow PRDX1 to gain entry to the cell nucleus. Once in the nucleus, PRDX1 promoted the transcription of LINC00460 and created a positive feedback system.
PRDX1 was also found to enrich promoters of genes related to the epithelial-to-mesenchymal transition such as ZEB1, ZEB2, and VIM. In this way, PRDX1 and LINC00460 worked together to facilitate the transition from epithelial to mesenchymal cells in a dependent manner. Further, high levels of LINC00460 and PRDX1 expression seemed to be associated with lymph metastasis, pathologic differentiation, and tumor size in patients with head and neck squamous cell carcinomas.
Disclosure: The study authors reported no conflicts of interest.